In the Altai region, researches on the industrial use of sea-buckthorn berries began almost simultaneously with the cultivation of the plant. Nowadays, sea buckthorn covers enough acreage to allow for its industrial pro- cessing. There have been in vitro and in vivo studies of sea-buckthorn products (juices, jam, oil, etc.) on humans and animals. These nutrition and pharmaceutical pro- ducts proved to have an anti-inflammatory, antitumoural, and antisclerotic effect on a living organism [1, 2]. As a rule, such preventive and therapeutic effect is attributed to phenol, vitamins, mineral substances, amino acids, fatty acids, and phitosterols. Sea buckthorn contains up to 11 satureted as well as mono- and polyunsarurated fatty acids. In addition, the berries contain α- and γ-to- copherols and α-tocotrienol, as well as some phitosterols, including campesterol, β-sitosterol, ∆5-avenasterol, cy- cloartenol, and gramisterol, which have a strong antio- xidant effect [3, 4]. Sea-buckthorn berries are known to contain a large amount of cartienoids and their ethers, such as astaxanthin, zeaxanthin,  zeaxanthin-palmitate, α-, β-, and γ-carotenes, cis-β-carotene, β-cryptoxanthin, lycopene, lutein-palmitate-myristate, and other biologi- cally active compounds [5–9].Nevertheless, the huge potential of sea-buckthorn is hardly used for fruit wine production because of a high oil content in sea-buckthorn berries. Thus, the berries are difficult to process, and the resulting drinks are sensory unstable [10].According to the previous research [11], the low sta- bility of sea buckthorn wine is probably connected with high-reactive substances of the phenol origin in its com- position. The substances are prone to copolymerization and condensation reactions; as a result, the drinks tend to be of dark colour. A high concentration of phenol sub- stances proved to be an essential feature of sea-buckthorn berries. Sea-buckthorn flavonoids are represented by catechins, leucoanthocyanins, prosyanidins, flavan-3-ol, and, to a lesser extent, by flavones. Also, the berries con-  Copyright © 2019, Shkolnikova et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material for any purpose, even commercially, provided the original work is properly cited and states its license.   tain coumarins and tanning substances [12–16]. Never- theless, the polyphenols are able to inhibit the formation of Maillard reaction products. Presumably, the mecha- nism can be explained by the fact that some polyphenols might interact with the intermediate products of the Mail- lard reaction: polyphenolic amides obstruct the reaction and result in sugar and amino acid degradation products of dark colour.The phenol compounds of grape and fruit wines af- fect such sensory properties as colour and taste [18–20]. Excessive phenol compounds make white wines rough and harsh. Usually, the rough taste is attributed to the tanning substances [21–23], e.g. prosyanidins. The ef- fect of polyphenols on the colour of white wines is deter- mined by both enzymic and nonenzymic oxidation when exposed to oxygen. As a result, the wine acquires amber colour, which may turn dark-brown if exposed to oxygen for too long. Such changes of colour are inappropriate for table wines [20].Activated  carbon  can  improve  the  sensory  charac- teristics of sea-buckthorn wines. In fact, activated car- bon of Granucol series is often used to improve the taste and colour of grape wine [32, 33]. This brand of carbon can be used for different technological purposes. For in- stance, Granucol GE adsorbs unwanted taste and smell; Granucol FA is used to remove the reddish tint in young wine; Granucol BI can lower the amount of phenol and monomer substances. Fruit wine industry has developed a lot of ways to improve such indices of must as sugari- ness or acidity. However, there are little experimental data on how to lower and stabilize polyphenols, in spite of the fact that it is polyphenols that are responsible for the harsh and rough taste, as well as browning during storage. Eye appeal is an important aspect that determines the reaction of customers when they choose wines and winy beverages of an unfamiliar trademark [34]. Thus, com- petitiveness requires that local wines should be attractive without losing their shelf stability. Appearance can be objectively assessed by analysing chromatic character- istics, e.g. colour intensity, tint, and coordinates in the CIE Lab system [35–40]. By determining the chromatic properties of wine and winy beverages, it is also possible to measure its yellowness, since yellowness has recent- ly been introduced into control practice for many nutri- tion products. It characterizes the change in colour of atest sample from clear or white toward yellow [41–43].The research objective was to analyse the effect var- ious amounts of Granucol activated carbon produce on the polyphenols content and the chromatic and sensory characteristics of dry sea-buckthorn wine.The research featured dry wine materials of sea buckthorn (Novost Altaya variety) harvested in 2014 in Barnaul at M.A. Lisavenko Research Institute of Sibe- rian Gardening. The initial amount of polyphenols was 480 ± 4.5 mg/dm3. The wine materials were produced by submerged cap fermentation with the help of Oenofermyeast, race LW 317-28 (Erbslöh Geisenheim AG, Germa- ny). Clarification of the wine material was performed us- ing 2.0–2.5 g/dm3 of bentonite. The final filtration of the wine materials was made with the help of a SEITS-KS80 filter-paperboard. The ageing time of the wine materi- al was 42 weeks at 5 ± 1°C. The general amount of SO was 80 mg/dm3. Granucol carbon (Erbslöh Geisenheim AG, Germany) was applied in rising concentrations from 10 to 150 mg/100 ml at 10 mg/100 ml intervals. 2The mass concentration of general phenolic substance was determined according to the colourimetric method with the Folin-Ciocalteu reagent [44–46] on the spec- trophotometer PE-5300VI (Ecros, Russia). The samples were preliminary diluted by 100.The optic and chromatic characteristics of the sam- ples before and after activated carbon treatment were determined in accordance with the methodic recommen- dations compiled by the OIV [47, 48] with the help of a UV-1800 spectrophotometer (Shimadzu, Japan).Based on the spectral characteristics of the wine ma- terials, we calculated:the value of colour intensity (I) represented by the sum of absorption values of the wine materials at the wave lengths of 420, 520, and 620 nm:I = A420  + A520  + A620;                       (1)the value of wine material colour tint (N) represented by the ratio of absorption value at the wave lengths of 420 and 520 nm: N = A420     A520 ;                            (2)the value of yellowness (G, %) according to the formu- la introduced in [49]:(1.28X - 1.06Z )100 G =                                      ,                      (3)Ywhere X, Y, and Z are coordinates of colour in the CIE system:X = 0.42 × T625  + 0.35 × T550  + 0.21× T445, (4) Y = 0.20 ×T625 + 0.63×T550 + 0.17 ×T495, (5) Z = 0.24 × T495 + 0.94 × T445,             (6) 625       550       445                   495where T , T , T  , and T  are coefficients of transmit- tance relative to distilled water at the corresponding wave lengths, %.To analyse the effect of Granucol carbons on the sensory characteristics of sea-buckthorn wines, different amounts of the activated carbon were added into the pro- cessed and aged wine materials and stirred for two hours. Finally, the wine was filtered from carbon. After that, the samples were tested for mass concentration of polyphe- nols and the optic characteristics of wine materials.RESULTS AND DISCUSSIONFig. 1 shows the dynamic changes in the amount of the phenolic compounds in the wine material according to the concentration and type of Granucol carbon.Fig. 2 shows that the usage of Granucol carbon re-duced  the  polyphenol  concentration  in  the  sea-buck-    The mass concentration of phenolic substances (expressed as gallic acid), mg/dm3 500  450 400 350 300 250 200   10       30       50       70       90      110     130     150The mass concentration of carbon, mg/100 cm3  1.16  Colour intensity 1.01  0.86 0.71 0.56  10     25     40     55     70     85    100   115   130   145Mass concentration of carbon, mg/100 cm3   Granucol BI             Granucol FA             Granucol GE Fig. 1. Effects of the mass concentration of the phenolic substances in the sea-buckthorn wine material on the concen- tration and type of Granucol carbon. thorn wine material. Granucol BI demonstrated the best results. In general, this type of carbon helped lower the amount of phenolic substances in the sea-buckthorn wine by 2.1 times when the maximum carbon amount was 150 mg/100 ml. Granucol FA and Granucol GE also low- ered the amount of polyphenols. However, they were less effective and reduced the amount of polyphenols only by1.52 and 1.56 times, respectively. Fig. 2 shows the em- piric isotherms of phenolic substances adsorption by dif- ferent activated Granucol carbons.We calculated the specific adsorption by the follow- Tinting formula:  Granucol BI              Granucol FA              Granucol GE  Fig. 3. Effects of the concentration and type of Granucol car- bons on the colour intensity.carbon absorbs the phenolic substances that exhaust the media due to their monomer nature.The optical properties of wine material help deter- mine its quality, age, and technological peculiarities. For instance, one can define the age and composition by the colour of wine. Any deviations from the colour norm mean that the wine in question is defective.A Shimadzu UV-1800 spectrophotometer was used   3.10 С  - С А =     0т  ×V ,                                 (7) 2.90  0 where Cis the mass concentration of phenolic substanc- 2.70 es in the starting wine material, mg/dm3;C is the mass concentration of phenolic substances in the processed wine material, mg/dm3;m is the mass of the used sorbent, mg; and  2.50   10              40              70             100            130Mass concentration of carbon, mg/100 cm3 V is the volume of the processed solution, dm3.Here we can see that the most effective concentration of Granucol BI was 20–60 mg/dm3. Probably, this type of  Specific absorption of phenolic substances by Granucol carbons, mg/mg 0.5  Granucol BI             Granucol FA             Granucol GE  Fig. 4. Effects of the concentration and type of Granucol carbons on the tint of the wine material.   0.4                                                                                                  680.3                                                                                                  61  Yellowness 0.2                                                                                                  54   0.1 0.0     10       30       50       70       90      110     130     150Mass concentration of carbon, mg/100 cm3 47 40 3310        30        50        70        90       110      130      150  Granucol BI            Granucol FA            Granucol GE Fig. 2. Isotherms of adsorption of phenolic substances in sea-buckthorn wine material by different types of Granucol carbons. Mass concentration of carbon, mg/100 cm3 Granucol BI          Granucol FA           Granucol GE  Fig. 5. Effects of concentration and type of Granucol carbon on the yellowness of the sea-buckthorn wine material. Colour coordinate y 0.40  0.40     Colour coordinate x 0.38  0.39    0.36 0.39 0.38   0.34   10              40              70             100             130Mass concentration of carbon, mg/100 cm3 0.38    10              40              70             100            130 Mass concentration of carbon, mg/100 cm3  Granucol BI             Granucol FA             Granucol GE Granucol BI             Granucol FA             Granucol GEFig. 6. Effects of the concentration and type of Granucol carbon on the displacement of the coordinates X and Y (according to the CIE 1931 chromatic system of coordinates).  to measure the optical density of the wine material in cuvettes with a path length of 10 mm. To define the in- tensity and tint of the colour, the optical density was measured at the waves of 420 and 520 nm. To obtain the trichromatic coordinates, we calculated the transmittance at 445, 495, 520, and 650 nm. The results were calculat- ed according to the OIV methods [41, 42].The following dependency graphs feature the colour intensity, tint, and yellowness according to the concentra- tion of Granucol carbons (Fig. 3, 4).The physical-and-chemical analysis and simple vi- sual observation proved that Granucol carbon lowered the colour intensity. A larger mass of Granucol carbon changed the colour of the wine material from intense am- ber to light yellow. Granucol FA and Granucol GE also reduced the intensity of colour. However, the wines vi- sually maintained the brown tint, which made them less attractive.Yellowness is  another factor  that  characterises the state of wine and wine materials, but fixed  standards have been established for grape wines only [43]. Cur- rently, yellowness is not used for sea-buckthorn wines assessment or for fruit wines in general. Nevertheless, we calculated the index of yellowness of our samples. Fig. 5 shows the changes of yellowness according to the con- centration and type of Granucol carbon.Remarkably, Granucol BI proved to be the most ef- fective type of carbon to improve the wine colour: not only did it lower the amount of phenolic substances, but it also improved it by making the wine more visually at- tractive. Granucol FA and Granucol GE also improved the colour and removed partly the brown tint, but their amounts were higher.The trichromatic colour coordinates of wine (xyz) and the subsequent coordinates X and Y were calculated ac- cording to the CIE Lab system of coordinates. Granucol carbon changed the coordinate X (the chromatic green- red axis) and produced almost no change on the coordi- nate Y (the chromatic yellow-blue axis) (Fig. 6a and 6b).The beneficial effect of Granucol carbon on the aro-ma and taste were also quite remarkable (Fig. 7).CONCLUSIONSThe present research proved that the activated carbon of the Granucol series can improve the sensory properties (taste and colour) of sea-buckthorn wine. The experiment demonstrated the effect of the concentration of carbons on the chromatic properties of wine. Granicol BI proved to be the most effective type of carbon to remove brown- ing caused by oxidation, and Granucol GE greatly im- proved the sensory perception of taste and aroma. CONFLICT OF INTERESTThe authors declare no conflict of interest related tothis article. FUNDINGThe research was financed by the administration of Biysk Technological Institute (branch) of the Polzunov Altai State Technical University.