Foods and Raw Materials Foods and Raw Materials Foods and Raw Materials 2308-4057 2310-9599 53434 10.21603/2308-4057-2023-1-547 Research Article Research Article Research Article Pig adipose tissue of two different breeds and locations: morphology and Raman studies Pig adipose tissue of two different breeds and locations: morphology and Raman studies https://orcid.org/0000-0001-8923-8661 Pchelkina Viktoriya A. Pchelkina Viktoriya A. v.pchelkina@fncps.ru https://orcid.org/0000-0003-4298-0927 Chernukha Irina M. Chernukha Irina M. https://orcid.org/0000-0002-8313-4105 Nikitina Мarina А. Nikitina Marina A. https://orcid.org/0000-0002-7980-3193 Ilin Nikolai A. Ilin Nikolai A. V.M. Gorbatov Federal Research Center for Food Systems of RAS Moscow Россия V.M. Gorbatov Federal Research Center for Food Systems of RAS Moscow Russian Federation Russian Biotechnological University (ROSBIOTECH) Moscow Россия Russian Biotechnological University (ROSBIOTECH) Moscow Russian Federation V.M. Gorbatov Federal Research Center for Food Systems of RAS Moscow Россия V.M. Gorbatov Federal Research Center for Food Systems of RAS Moscow Russian Federation V.M. Gorbatov Federal Research Center for Food Systems of RAS Moscow Россия V.M. Gorbatov Federal Research Center for Food Systems of RAS Moscow Russian Federation V.M. Gorbatov Federal Research Center for Food Systems of RAS Moscow Россия V.M. Gorbatov Federal Research Center for Food Systems of RAS Moscow Russian Federation 17 03 2023 17 03 2023 11 1 1 9 25 08 2022 04 10 2022 https://jfrm.ru/en/issues/20672/20669/

According to the recent data, there are 4–5-local pig breeds left in Russia by now. Livni is among them. This breed is characterized by high fat content. Back fat has been analyzed earlier. We aimed to assess fat morphometrics from other localizations in pigs. Sacral, axillary, and perirenal fat samples from 6-month-old Duroc and Livni pig breeds were analyzed using morphological and Raman-based techniques. Livni adipocytes were characterized by dense packing with a polyhedron-like structure. In Duroc fat, they were more rounded (spherical). A “two-phase” cell disperse was identified in all samples. Fat cells in Livni pigs were bigger than those in the Duroc breed: 70–102, 15–18, and 26% for sacral, axillary, and perirenal locations. Differences in the intensity of the Raman signal between the samples were found: in the samples of subcutaneous adipose tissue, more intense peaks were observed, which are responsible for unsaturation; the samples of Livni axillary fat were characterized by greater unsaturation than sacral fat. Livni and Duroc adipocytes differ from each other in form and size and the difference depends on location. Pork fat from local breeds is expected to have potentially more health protecting (for animals) and health promoting (for consumers) properties.

According to the recent data, there are 4–5-local pig breeds left in Russia by now. Livni is among them. This breed is characterized by high fat content. Back fat has been analyzed earlier. We aimed to assess fat morphometrics from other localizations in pigs. Sacral, axillary, and perirenal fat samples from 6-month-old Duroc and Livni pig breeds were analyzed using morphological and Raman-based techniques. Livni adipocytes were characterized by dense packing with a polyhedron-like structure. In Duroc fat, they were more rounded (spherical). A “two-phase” cell disperse was identified in all samples. Fat cells in Livni pigs were bigger than those in the Duroc breed: 70–102, 15–18, and 26% for sacral, axillary, and perirenal locations. Differences in the intensity of the Raman signal between the samples were found: in the samples of subcutaneous adipose tissue, more intense peaks were observed, which are responsible for unsaturation; the samples of Livni axillary fat were characterized by greater unsaturation than sacral fat. Livni and Duroc adipocytes differ from each other in form and size and the difference depends on location. Pork fat from local breeds is expected to have potentially more health protecting (for animals) and health promoting (for consumers) properties.

 Fat pigs Duroc Livni histology adipocytes morphometry Raman spectroscopy Fat pigs Duroc Livni histology adipocytes morphometry Raman spectroscopy This work was financially supported by the Russian Science Foundation (RSF) (project No. 21-76-20032). This work was financially supported by the Russian Science Foundation (RSF) (project No. 21-76-20032).

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