Foods and Raw Materials Foods and Raw Materials Foods and Raw Materials 2308-4057 2310-9599 73656 10.21603/2308-4057-2024-2-615 Research Article Research Article Research Article Properties of plant extracts and component composition: column chromatography and IR spectroscopy Properties of plant extracts and component composition: column chromatography and IR spectroscopy https://orcid.org/0000-0002-4921-8997 Babich Olga O. Babich Olga O. https://orcid.org/0000-0001-5026-7510 Samsuev Ilya G. Samsuev Ilya G. https://orcid.org/0000-0001-8578-0701 Tcibulnikova Anna V. Tcibulnikova Anna V. Zemlyakova Evgeniya S. Zemlyakova Evgeniya S. Popov Alexander D. Popov Alexander D. https://orcid.org/0000-0002-1252-9572 Ivanova Svetlana A. Ivanova Svetlana A. pavvm2000@mail.ru https://orcid.org/0000-0003-1198-1951 Noskova Svetlana Yu. Noskova Svetlana Yu. https://orcid.org/0000-0001-7910-8388 Sukhikh Stanislav A. Sukhikh Stanislav A. stas-asp@mail.ru Immanuel Kant Baltic Federal University Kaliningrad Россия Immanuel Kant Baltic Federal University Kaliningrad Russian Federation Immanuel Kant Baltic Federal University Kaliningrad Россия Immanuel Kant Baltic Federal University Kaliningrad Russian Federation Immanuel Kant Baltic Federal University Kaliningrad Россия Immanuel Kant Baltic Federal University Kaliningrad Russian Federation Kaliningrad State Technical University Kaliningrad Россия Kaliningrad State Technical University Kaliningrad Russian Federation Immanuel Kant Baltic Federal University Kaliningrad Россия Immanuel Kant Baltic Federal University Kaliningrad Russian Federation Kemerovo State University Kemerovo Россия Kemerovo State University Kemerovo Russian Federation Immanuel Kant Baltic Federal University Kaliningrad Россия Immanuel Kant Baltic Federal University Kaliningrad Russian Federation Immanuel Kant Baltic Federal University Kaliningrad Россия Immanuel Kant Baltic Federal University Kaliningrad Russian Federation 09 04 2024 09 04 2024 12 2 373 384 29 09 2023 09 01 2024 https://jfrm.ru/en/issues/22111/22272/

Medicinal plants contain various biologically active substances. This study aimed to investigate properties of plant extracts and component composition of plant raw materials from the continental part Kaliningrad region (Guards district). For this, we used column chromatography and IR spectroscopy. The objects of the study were samples of plant extracts of Eryngium maritimum, Hedysarum neglectum, Melilotus officinalis, and Aesculus hippocastanum. To produce medicinal plant extracts, we prepared methanol extraction by the Soxhlet method for 8 h (15 cycles). The antioxidant activity of the studied samples was determined by their ability to reduce the radical 2,2-diphenyl-1-picrylhydrazyl. The disk-diffusion method was used to evaluate the antimicrobial activity of the plant extracts against such test strains as Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans. In the extracts, 3,4-dihydroxybenzoic acid, astragalin, luteolin-7-glucoside, rosmarinic acid, and chlorogenic acid were identified. However, more research is needed to determine which of the individual phenolic compounds in E. maritimum, H. neglectum, M. officinalis, and A. hippocastanum are involved in exhibiting antioxidant activity. It was found that the plant extract of H. neglectum had activity against the bacterium B. subtilis and the mold fungus C. albicans, while the plant extract of E. maritimum was detrimental to the growth and development of both Gram-positive and Gram-negative bacteria. Infrared spectroscopy can help in further studies to determine properties of medicinal plants to ensure the safety and efficacy of plant-based products.

Medicinal plants contain various biologically active substances. This study aimed to investigate properties of plant extracts and component composition of plant raw materials from the continental part Kaliningrad region (Guards district). For this, we used column chromatography and IR spectroscopy. The objects of the study were samples of plant extracts of Eryngium maritimum, Hedysarum neglectum, Melilotus officinalis, and Aesculus hippocastanum. To produce medicinal plant extracts, we prepared methanol extraction by the Soxhlet method for 8 h (15 cycles). The antioxidant activity of the studied samples was determined by their ability to reduce the radical 2,2-diphenyl-1-picrylhydrazyl. The disk-diffusion method was used to evaluate the antimicrobial activity of the plant extracts against such test strains as Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans. In the extracts, 3,4-dihydroxybenzoic acid, astragalin, luteolin-7-glucoside, rosmarinic acid, and chlorogenic acid were identified. However, more research is needed to determine which of the individual phenolic compounds in E. maritimum, H. neglectum, M. officinalis, and A. hippocastanum are involved in exhibiting antioxidant activity. It was found that the plant extract of H. neglectum had activity against the bacterium B. subtilis and the mold fungus C. albicans, while the plant extract of E. maritimum was detrimental to the growth and development of both Gram-positive and Gram-negative bacteria. Infrared spectroscopy can help in further studies to determine properties of medicinal plants to ensure the safety and efficacy of plant-based products.

 Medicinal plants Eryngium maritimum Melilotus officinalis Hedysarum neglectum Aesculus hippocastanum extracts infrared spectroscopy antioxidant properties Medicinal plants Eryngium maritimum Melilotus officinalis Hedysarum neglectum Aesculus hippocastanum extracts infrared spectroscopy antioxidant properties This research was funded by the Russian Science Foundation (RSF), grant number 22-16-00044. This research was funded by the Russian Science Foundation (RSF), grant number 22-16-00044.

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