Moscow, Moscow, Russian Federation
from 01.01.2006 until now
Istra, Moscow, Russian Federation
Istra, Russian Federation
Moscow, Russian Federation
Istra, Russian Federation
The growing consumer interest in healthy foods increases the demand for new functional products. Mare’s milk and its products possess some unique nutritional properties. This article introduces a new fermented functional food that combines mare’s milk, cow’s milk, lactic acid, and probiotic microorganisms. The research featured fermented milk products based on mare’s milk, cow’s milk, and their mixes. The preclinical studies involved clinically healthy female mice (C57BL/6J). The biochemical analysis of blood plasma was performed on a BioChem FC-360 automatic analyzer while the histological preparations relied on an AxioImaiger A1 light microscope. The quantitative assessment employed the ImageJ software (USA). The mice obtained the so-called Western diet and the experimental product. Their low-density lipoprotein cholesterol (LDL-C) decreased by 254-30.0% while the high-density lipoprotein cholesterol (HDL-C) went up. The mice that were given the experimental product demonstrated lower creatinine, urea, and alanine aminotransferase activity in the blood plasma, which indicated a certain hepatoprotective effect. The experimental product caused a statistically significant decrease in granulocytes, i.e., the microbiome had an anti-inflammatory effect on the gut microbiota, which, in its turn, affected the cytokine expression. The new product demonstrated strong biological activity, which rendered it functional properties. Further research will determine the effect of fermented milk products as part of a comprehensive diet on non-alcoholic fatty liver disease.
Fermented milk product, mare’s milk, cow’s milk, probiotics, biological effects
1. Plamada D, Teleky B-E, Nemes SA, Mitrea L, Szabo K, Călinoiu L-F, et al. Plant-based dairy alternatives - A future direction to the milky way. Foods. 2023;12(9):1883. https://doi.org/10.3390/foods12091883
2. Zobkova ZS. Dependence of the relative biological value of fermented milk drinks on the type of starter microorganisms. Dairy Industry. 2020;(8):36–37. (In Russ.). https://doi.org/10.31515/1019-8946-2020-08-36-37; https://elibrary.ru/XZVCXA
3. Byberg L, Lemming E W. Milk Consumption for the Prevention of Fragility Fractures. Nutrients. 2020;12(9):2720. https://doi.org/10.3390/nu12092720.
4. Nestel PJ, Mellett N, Pally S, Wong G, Barlow CK, Croft K, et al. Effects of low-fat or full-fat fermented and non-fermented dairy foods on selected cardiovascular biomarkers in overweight adults. British Journal of Nutrition. 2013;110(12):2242–2249. https://doi.org/10.1017/S0007114513001621
5. Sonestedt E, Wirfält E, Wallström P, Gullberg B, Orho-Melander M, Hedblad B. Dairy products and its association with incidence of cardiovascular disease: the Malmö diet and cancer cohort. European Journal of Epidemiology. 2011;26:609–618. https://doi.org/10.1007/s10654-011-9589-y
6. Park YW. Goat Milk – Chemistry and Nutrition. In: Haenlein GF, Wendorff WL, Park YW, editors. Handbook of milk of non-bovine mammals. London: Blackwell; 2006; pp. 34–58. https://doi.org/10.1002/9781119110316
7. Czyżak-Runowska G, Wójtowski JA, Danków R, Stanisławski D. Mare’s milk from a small polish specialized farm - Basic chemical composition, fatty acid profile, and healthy lipid indices. Animals. 2021;11(6):1590. https://doi.org/10.3390/ ani11061590
8. Faccia M, D’Alessandro AG, Summer A, Hailu Y. Milk products from minor dairy species: A review. Animals. 2020; 10(8):1260. https://doi.org/10.3390/ani10081260
9. Baibokonov D, Yang Y, Tang Y, Hosain MdS. Understanding the traditional mares’ milk industry’s transformation into a creative industry: Empirical evidence from Kazakhstan. Growth and Change. 2021;52(2):1172–1196. https://doi.org/https://doi.org/10.1111/grow.12478
10. Sarsembayev KhS, Sinyavskiy YuA, Deripaskina EA, Tuigunov DN, Torgautov AS. Fermented dairy product for sports nutrition. Human. Sport. Medicine. 2022;22(1):148–154. (In Russ.). https://doi.org/10.14529/hsm220120; https:// elibrary.ru/ITTKAB
11. Simonenko ES, Simonenko SV, Zolotin AYu, Bessarab OV. Development of a methodology for assessing the organoleptic perception of a food product. Food Industry. 2021;(6):57–62. (In Russ.). https://doi.org/10.52653/PPI.2021.6.6.006; https://elibrary.ru/LCVZBC
12. Semenova ES, Simonenko ES, Manuilov BM, Kopytko MS. Mare’s milk is a promising raw material for creating baby food products with functional properties. Food Industry. 2022;(11):58–61. (In Russ.). https://doi.org/10.52653/PPI.2022.11.11.014; https://elibrary.ru/LDKRLO
13. Singh S, Sharma RK, Malhotra S, Pothuraju R, Shandilya UK. Lactobacillus rhamnosus NCDC17 ameliorates type-2 diabetes by improving gut function, oxidative stress and inflammation in high-fat-diet fed and streptozotocintreated rats. Beneficial Microbes. 2016;8(2):243–55. https://doi.org/10.3920/BM2016.0090
14. Navrátilová P, Pospíšil J, Borkovcová I, Kaniová L, Dluhošová S, Horáková S. Content of nutritionally important components in mare milk fat. Mljekarstvo. 2018;68(4):282–294. https://doi.org/10.15567/mljekarstvo.2018.0404
15. Francque SM, Van Der Graaff D, Kwanten WJ. Non-alcocholic fatty liver disease and cardiovascular risk: pathophysiological mechanisms and implications. Journal of Hepatology. 2016;65(2):425–443. https://doi.org/10.1016/j.jhep.2016.04.005
16. Dumas ME, Barton RH, Toye A, Cloarec O, Blancher C, Rothwell A, et al. Metabolic profiling reveals a contribution of gut microbiota to fatty liver phenotype in insulin-resistant mice. Proceedings of the National Academy of Sciences. 2006;103(33):12511–12516. https://doi.org/10.1073/pnas.0601056103
17. Targher G, Byrne CD, Lonardo A, Zoppini G, Barbui C. Non-alcoholic fatty liver disease and risk of incident cardiovascular disease: a meta-analysis. Journal of Hepatology. 2016;65(3):589–600. https://doi.org/10.1016/j.jhep.2016.05.013
18. Zhang, QQ, Lu L-G. Nonalcoholic fatty liver disease: dyslipidemia, risk for cardiovascular complications, and treatment strategy. Journal of Clinical and Translational Hepatology. 2015;3(1):74–78. https://doi.org/10.14218/JCTH.2014.00037
19. Katsiki N, Mikhailidis DP, Mantzoros CS. Non-alcoholic fatty liver disease and dyslipidemia: an update. Metabolism. 2016;65(8):1109–1123. https://doi.org/10.1016/j.metabol.2016.05.003
20. Fedulova LV. Theoretical validity and practical effectiveness of an integrated approach to the research of specialized food products. Dr. Tech. Sci. dis. Moscow: Federal Research Center for Food Systems named after. V.M. Gorbatova; 2021. 361 p. (In Russ.). https://elibrary.ru/FIDJOS
21. Chernukha I, Kotenkova E, Derbeneva S, Khvostov D. Bioactive compounds of porcine hearts and aortas may improve cardiovascular disorders in humans. International Journal of Environmental Research and Public Health. 2021;18(14):7330. https://doi.org/10.3390/ijerph18147330
22. Mehlem A, Hagberg CE, Muhl L, Eriksson U, Falkevall A. Imaging of neutral lipids by oil red O for analyzing the metabolic status in health and disease. Nature Protocols. 2013;8(6):1149–1154. https://doi.org/10.1038/nprot.2013.055
23. Abdel-Salam AM. Functional foods: Hopefulness to good health. American Journal of Food Technology. 2010; 5(2):86–99. https://doi.org/10.3923/ajft.2010.86.99
24. Lazo, M, Clark JM. The epidemiology of nonalcoholic fatty liver disease: a global perspective. In Seminars in liver disease.2008;28(04):339–350. https://doi.org/10.1055/s-0028-1091978
25. Perdomo G, Kim DH, Zhang T, Qu S, Thomas EA, Toledo FGS, et al. A role of apolipoprotein D in triglyceride metabolism [S]. Journal of lipid research. 2010;51(6):1298–1311. https://doi.org/10.1194/jlr.M001206
26. Ali K, Abo-Ali EM, Kabir MD, Riggins B, Nguy S, Li L, et al. A Western-fed diet increases plasma HDL and LDL-cholesterol levels in ApoD–/–mice. PLoS One. 2014;9(12):e115744. https://doi.org/10.1371/journal.pone.0115744
27. Rogero MM, Calder PC. Obesity, Inflammation, Toll-Like Receptor 4 and Fatty Acids. Nutrients. 2018;10(4):432. https://doi.org/10.3390/nu10040432
28. Zhang D, Frenette PS. Cross talk between neutrophils and the microbiota. Blood.2019;133(20):2168–2177. https:// doi.org/10.1182/blood-2018-11-844555
29. Aktas B, De Wolfe TJ, Tandee K, Safdar N, Darien BJ, Steele JL. The Effect of Lactobacillus casei 32G on the Mouse Cecum Microbiota and Innate Immune Response Is Dose and Time Dependent. PLoS ONE. 2015;10(12):e0145784. https://doi.org/10.1371/journal.pone.0145784
30. Ma J, Zhou Q, Li H. Gut Microbiota and Nonalcoholic Fatty Liver Disease: Insights on Mechanisms and Therapy. Nutrients. 2019;9(10):1124. https://doi.org/10.3390/nu9101124
