Kemerovo, Russian Federation
Kemerovo, Russian Federation
from 01.01.2018 to 01.01.2020
Kemerovo, Russian Federation
Kemerovo, Russian Federation
Milk fermentation and cheese sensory profiles depend on microbial activities. Probiotic bacterial strains improve cheese quality and impart specific functional properties to the final product. This article describes the effect of microbial consortia based on Enterococcus faecium JDC 86 (B-4054), Lactobacillus acidophilus 13 (B-2585), and Lactobacillus plantarum B (B-11264) on cheese. The study evaluated the following parameters: antibiotic resistance, antimicrobial activity against Escherichia coli, NaCl tolerance, milk-curdling ability, and acidification. The B-4054:B-11264 (1:1 ratio) and B-2585:B-11264 (1:1 ratio) consortia proved to be antibiotic-resistant and exhibited antimicrobial activity against E. coli while maintaining milk-curdling and acidification capabilities. The B-4054:B-11264 consortium demonstrated the highest sensitivity to NaCl: 2.0% NaCl in the nutrient medium decreased the biomass (in optical density units) by a factor of 1.5, 4.0% NaCl by a factor of 6.3, and 6.5% NaCl by a factor of 18.4. In contrast, 2.0% NaCl reduced the biomass of the B-2585:B-11264 consortium by a factor of 1.01, 4.0% NaCl by a factor of 1.7, and 6.5% NaCl by a factor of 8.5. These consortia showed great promise for functional cheese production but require further research.
microorganisms, consortium, acid formation, fermenting ability, antibiotic resistance, antimicrobial activity, cheese
1. Vesnina, A. D. Gut microbiota and its role in development of chronic disease and aging / A. D. Vesnina [et al.] // Foods and Raw Materials. 2026. Vol. 14(1). P. 174–197. https://doi.org/10.21603/2308-4057-2026-1-668
2. Settanni, L. Non-starter lactic acid bacteria used to improve cheese quality and provide health benefits / L. Settanni, G. Moschetti // Food Microbiology. 2010. Vol. 27(6). R. 691–697. https://doi.org/10.1016/j.fm.2010.05.023
3. Ouwehan, A. S. Probiotics: an overview of beneficial effects / A. C. Ouwehan, S. Salminen, E. Isolauri // Antonie van Leeuwenhoek. 2002. Vol. 82(1–4). R. 279–289. 4. Shenderov, B. A. Innovacionnye produkty i ingredienty-drayvery molochnogo rynka / B. A. Shenderov // Molochnaya promyshlennost'. 2013. № 6. S. 62–66. https://elibrary.ru/qbxoeb
4. Leroy, F. Lactic acid bacteria as functional starter cultures for the food fermentation industry / F. Leroy // Trends in Food Science & Technology. 2004. R. 67–78. https://doi.org/10.1016/J.TIFS.2003.09.004
5. Stoyanova, L. G. Metabioticheskie svoystva shtammov Lactobacillus acidophilus, vhodyaschih v kompleksnye zakvaski dlya proizvodstva probioticheskih molochnyh produktov / L. G. Stoyanova, S. D. Dbar, I. S. Polyanskaya // Biotehnologiya. 2022. T. 38. № 1. S. 3–12. https://doi.org/10.56304/S0234275822010070; https://elibrary.ru/bimhwp
6. Ganesan, B. Probiotic bacteria survive in Cheddar cheese and modify populations of other lactic acid bacteria / B. Ganesan [et al.] // Journal of applied microbiology. 2014. Vol. 116(6). P. 1642–1656. https://doi.org/10.1111/jam.12482
7. Semenihina, V. F. Biotehnologiya kislomolochnyh produktov i preparatov s probioticheskimi svoystvami / V. F. Semenihina [i dr.] // Molochnaya promyshlennost'. 2016. № 7. S. 57–58. https://elibrary.ru/wayuzn
8. Frolova, A. S. Potencial molochnokislyh bakteriy-probiotikov s perspektivoy ih ispol'zovaniya v syrodelii / A. S. Frolova [i dr.] // Syrodelie i maslodelie. 2025. № 4. S. 70–76. https://doi.org/10.21603/2073-4018-2025-4-35; https://elibrary.ru/zupiug
9. Dapkevicius, M. De. L. E. Current trends of enterococci in dairy products: A comprehensive review of their multiple roles / M. De. L. E. Dapkevicius [et al.] // Foods. 2021. Vol. 10(4). Art. no. 821. https://doi.org/10.3390/foods10040821
10. Suhodolec, V. V. Molochnokislye enterokokki Enterococcus faecium i Enterococcus durans: raznoobrazie v posledovatel'nostyah nukleotidov v genah 16S rRNK / V. V. Suhodolec [i dr.] // Mikrobiologiya. 2005. № 6(74). S. 810–815. https://elibrary.ru/hsjjwj
11. Vesnina, A. D. Poluchenie probioticheskogo konsorciuma na osnove vydelennyh iz korov'ego moloka shtammov / A. D. Vesnina [i dr.] // Molochnohozyaystvennyy vestnik. 2021. № 2(42). S. 107–122. https://doi.org/10.52231/2225-4269_2021_2_107; https://elibrary.ru/yxajjk
12. Zare, D. The benefits and applications of Lactobacillus plantarum in food and health: A narrative review / D. Zare [et al.] // Iranian journal of public health. 2024. Vol. 53(10). R. 2201–2213. https://doi.org/10.18502/ijph.v53i10.16698
13. Gueimonde, M. Antibiotic resistance in probiotic bacteria / M. Gueimonde [et al.] // Frontiers in microbiology. 2013. Vol. 4. Art. no. 202. https://doi.org/10.3389/fmicb.2013.00202
14. Artyuhova, S. I. Znachenie issledovaniy prirodnoy ustoychivosti molochnokislyh bakteriy i bifidobakteriy k antibiotikam pri razrabotke novyh vidov bioproduktov / S. I. Artyuhova, G. S. Ponochevnaya, A. A. Sveshnikova // Mezhdunarodnyy zhurnal eksperimental'nogo obrazovaniya. 2014. № 8-1. S. 96–97. https://elibrary.ru/shhrwx
15. Kishilova, S. A. Sovremennye biotehnologicheskie resheniya v oblasti ispol'zovaniya molochnokislyh bakteriy dlya molochnoy promyshlennosti: ot selekcii shtammov do probioticheskih produktov / S. A. Kishilova [i dr.] // Tehnika i tehnologiya pischevyh proizvodstv. 2025. T. 55, № 3. S. 624–633. https://doi.org/10.21603/2074-9414-2025-3-2596; https://elibrary.ru/zvwsdn
16. Mironenko, I. M. Myagkie syry. Assortiment i tehnologicheskie osobennosti / I.M. Mironenko, D.A. Usatyuk // Syrodelie i maslodelie. 2015. № 4. S. 36–40. https://elibrary.ru/ubvtxf
17. Borovik, T. E. Skvashennye molochnye produkty v pitanii detey rannego vozrasta v Rossiyskoy Federacii: proshloe i nastoyaschee / T. E. Borovik [i dr.] // Voprosy sovremennoy pediatrii. 2016. T. 15, № 6. S. 556–561. https://doi.org/10.15690/vsp.v15i6.1651; https://elibrary.ru/xehokl
18. Casarotti, S. N. Influence of the combination of probiotic cultures during fermentation and storage of fermented milk / S. N. Casarotti [et al.] // Food Research International. 2014. Vol. 59. R. 67–75. https://doi.org/10.1016/j.foodres.2014.01.068
